FastQCFastQC Report
Mon 5 Sep 2022
M49_S139_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM49_S139_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6332355
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTCACAATA110900.17513231649204758No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG99170.15660840240321333No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC95090.15016530185057533No Hit
GACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCACCTTAA92680.14635945078884555No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC83060.13116763036816476No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC82490.13026749132036974No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT80680.1274091550457926No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG78790.12442448346626177No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG74480.11761816891188191No Hit
GTCGTGGACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCA71270.11254896480061526No Hit
GGGGACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTCACA70680.11161724192658182No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA70370.11112769261988628No Hit
GTGGACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCACCT68850.10872732182576625No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG67450.10651645398907672No Hit
GTCGGGGACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTC66040.104289794239268No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT65430.10332648753899616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)