FastQCFastQC Report
Mon 5 Sep 2022
M49_S139_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM49_S139_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203650
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTCACAATA56870.17751627050395644No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG50300.1570084122797434No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC47970.14973545799322646No Hit
GACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCACCTTAA46540.145271799353862No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC42270.13194325222792752No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC41310.12894667020429823No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT40790.12732352160816568No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG40450.12626223214146365No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG38230.11933263621182089No Hit
GTCGTGGACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCA36790.11483776317637695No Hit
GGGGACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTCACA36050.11252789786649603No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA35800.11174753796450922No Hit
GTGGACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCACCT35020.10931281507031043No Hit
GTCGGGGACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTC34550.10784573845457526No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG34450.10753359449378053No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT32840.10250807672498558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)