FastQCFastQC Report
Mon 5 Sep 2022
M49_S139_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM49_S139_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128705
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTCACAATA54030.17269125724540985No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG48870.15619881069004588No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC47120.15060544218774222No Hit
GACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCACCTTAA46140.14747315582645215No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC41750.1334417914121018No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC40220.12855158923580204No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT39890.1274968397467962No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG38340.12254271335904153No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG36250.11586263326200458No Hit
GGGGACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTCACA34630.11068477213415774No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA34570.11049299949979305No Hit
GTCGTGGACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCA34480.110205340548246No Hit
GTGGACCCACTCAATGTAAAATAATAGTTTCATATTTAAATTATGCACCT33830.1081278036759618No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG33000.10547494890058348No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT32590.10416450256575803No Hit
GTCGGGGACCCTGTAAGTAAATGAGTTTATGTATCACGTGTTTATGTTTC31490.10064867093573858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)