FastQCFastQC Report
Mon 5 Sep 2022
M48_S137_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM48_S137_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4592820
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC136000.29611436982072015No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG129450.281852979215384No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC129200.28130865132968413No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT113500.24712486010773338No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA101770.22158499571069626No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA87370.19023170949438473No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC71010.15461089265418632No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG56130.12221249689733105No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG52420.11413467107354523No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT49450.10766805579143097No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA48420.10542542490234758No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG45950.10004746539163303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)