FastQCFastQC Report
Mon 5 Sep 2022
M45_S140_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM45_S140_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5003497
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC92720.185310393910499No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC86770.17341871095355907No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG86190.17225952169052963No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC77580.15505155694107542No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT69880.1396623201732708No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG68220.13634464055839346No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA65150.130208931873048No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA58730.1173779058926187No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA54490.10890383265943798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)