FastQCFastQC Report
Mon 5 Sep 2022
M45_S140_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM45_S140_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522432
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC47180.18704171212544085No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC43940.17419696546824653No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG43150.17106506736356025No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC38780.15374051708827038No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT34920.1384378250830944No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG34870.13823960368406363No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA32910.1304693248420572No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA30170.11960679217517063No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA27600.10941821226498871No Hit
GCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACCCAACCTCTC25570.10137042346433918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)