FastQCFastQC Report
Mon 5 Sep 2022
M44_S163_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM44_S163_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095226
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG36060.17210553897288408No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC35620.17000552685008682No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC32170.15353952270542653No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT30530.1457122047931822No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA29570.14113036016162459No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA25960.12390071524503801No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG21540.10280513892057468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)