FastQCFastQC Report
Mon 5 Sep 2022
M43_S160_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM43_S160_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262470
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG115290.21907963370812567No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC111910.21265679424300757No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC108140.20549285791652971No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG106790.20292752262720737No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC102860.19545954656273573No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG97350.1849891780855758No Hit
CCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGG94440.17945945535081434No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT93640.1779392566608456No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA84950.1614260983910597No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA84090.15979188479934325No Hit
GGCCCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCAT82840.15741657434626705No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA79710.1514687969717642No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT77630.1475162803778454No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA76910.1461481015568735No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG75610.14367777868567422No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC74370.1413214707162226No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG72790.1383190783035343No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT70050.1331123977903912No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG67620.12849479426961105No Hit
GCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACCCAACCTCTC65130.1237631758470832No Hit
GTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCT61200.1162951997826116No Hit
AGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACATCCAAGG59360.1127987427956834No Hit
AACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTC56870.10806712437315556No Hit
AACACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGG54540.1036395456886215No Hit
TGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTC54270.10312647863075704No Hit
ACGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTT54220.103031466212634No Hit
TAACAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCAC53380.10143525758816677No Hit
GGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACAT53150.10099820046480075No Hit
ATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACATCCA53120.10094119301392691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)