FastQCFastQC Report
Mon 5 Sep 2022
M42_S169_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM42_S169_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941484
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC83290.16855260484502227No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC76720.15525700376647986No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG76560.1549332143947041No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC73220.148174111258885No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG70840.14335774435372048No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA65610.1327738792638001No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT61390.12423393458321427No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA61070.12358635583966274No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT60670.12277688241022333No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG58840.11907354147053799No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG57740.1168474895395796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)