FastQCFastQC Report
Mon 5 Sep 2022
M41_S161_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM41_S161_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5171338
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC82220.15899173482762102No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC75150.14532022466912817No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC70190.1357288964674133No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG69190.13379516094287397No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT61560.11904075889063914No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG60370.11673961361643737No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG58500.11312352818554888No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA54910.10618141765245281No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA54830.10602671881048967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)