FastQCFastQC Report
Mon 5 Sep 2022
M41_S161_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM41_S161_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562509
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC38860.15164824786956846No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC36990.14435071252432674No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC34390.13420440669671793No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG34090.13303367910122463No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT29440.1148874013710781No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG29270.11422398906696524No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG28470.11110204881231638No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA27640.10786303579811818No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA27400.10692645372172352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)