FastQCFastQC Report
Mon 5 Sep 2022
D58_S144_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD58_S144_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7576491
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC81150.10710763069605705No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC78020.10297643064579633No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC76930.10153776992541798No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG76310.10071944914868901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)