FastQCFastQC Report
Mon 5 Sep 2022
D57_S143_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD57_S143_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5124271
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG100790.19669139278543232No Hit
AAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAATAAT87140.17005345736008107No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC85680.1672042715929739No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT80280.1566661872488789No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC76170.14864553416476217No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG74520.14542556394851092No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC74150.14470351002123036No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG71380.13929786305212974No Hit
AGTGCCATATACCCCCCACATGAACTCTTGAGTACTAGATGAATGACTAT69870.1363511024299847No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA62410.12179293405832751No Hit
CCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAAT61140.11931453274036444No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT60100.11728497575557575No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG59560.11623116732116627No Hit
GGTCCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAG56980.11119630480120976No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA55810.10891305319332253No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA53450.10430752003553286No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT53330.10407334038344186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)