FastQCFastQC Report
Mon 5 Sep 2022
D57_S143_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD57_S143_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587500
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG50840.1964830917874396No Hit
AAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAATAAT44320.17128502415458938No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC43670.16877294685990338No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT40740.15744927536231884No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC38800.14995169082125603No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC38260.1478647342995169No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG37380.14446376811594203No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG35830.13847342995169082No Hit
AGTGCCATATACCCCCCACATGAACTCTTGAGTACTAGATGAATGACTAT35120.13572946859903381No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA31720.12258937198067632No Hit
CCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAAT31120.12027053140096619No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT30380.11741062801932367No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG30300.11710144927536233No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA28920.11176811594202898No Hit
GGTCCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAG28650.11072463768115942No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT27270.10539130434782608No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA26960.10419323671497585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)