FastQCFastQC Report
Mon 5 Sep 2022
D57_S143_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD57_S143_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2536771
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG49950.19690385927622162No Hit
AAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAATAAT42820.1687972623465027No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC42010.16560422679067208No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT39540.15586743935499103No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC37910.14944194805128252No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG37140.14640659326364105No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG35550.1401387827281217No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC35350.13935037888717586No Hit
AGTGCCATATACCCCCCACATGAACTCTTGAGTACTAGATGAATGACTAT34750.13698516736433838No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA30690.12098056939313798No Hit
CCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAAT30020.11833941652596944No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT29720.11715681076455069No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG29260.11534348193037527No Hit
GGTCCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAG28330.11167740406997716No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA26890.10600089641516716No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA26490.1044240887332755No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT26060.10272902047524196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)