FastQCFastQC Report
Mon 5 Sep 2022
D54_S145_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD54_S145_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115745
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAATAAT41920.19813351798066403No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG33920.16032177790801821No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC32720.15465001689712135No Hit
CCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAGAAT30150.1425029953987839No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT28300.13375903050698454No Hit
GGTCCGAAGAATGGGCCCTGTCACACGGCTTCATTGGAAGCTAAATACAG28000.13234109025426033No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG26710.1262439471675462No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG26650.12596035911700132No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC26330.12444788951409551No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA25010.11820895240210894No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC22600.10681816570522441No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA22140.10464399065104728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)