FastQCFastQC Report
Tue 6 Sep 2022
R59_S202_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR59_S202_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632388
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC108710.29927970249874186No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG105870.2914611544801932No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC89120.24534823922995008No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT84160.23169331029614676No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA80470.22153470389176486No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA74130.20408062134331467No Hit
CAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCACCTC44310.12198586714855353No Hit
TAACAGGCTAATCCTTTAGTAGAGTTCGTATTGGCTAAAGGGATTGGCAC43480.119700868960034No Hit
CACCTCGATGTTGAATCAGGGATAATAGCTTCAAGGCGTAGAGGCTTTGA43180.11887496600032817No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG36510.10051239019620149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)