FastQCFastQC Report
Tue 6 Sep 2022
R42_S175_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR42_S175_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4762742
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC86020.18061024510670534No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG77930.16362423158760225No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC77570.1628683644841564No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG73160.15360899246694446No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT67080.14084323694208084No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC65030.13653899371412517No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA63690.13372548838463222No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT56920.11951098757816402No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA55290.11608858930422852No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG54700.11484980710691446No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA52750.11075552696324933No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG49330.10357478948051355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)