FastQCFastQC Report
Tue 6 Sep 2022
R41_S181_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR41_S181_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5982200
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC139170.23264016582528166No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG138480.23148674400722144No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC123420.2063120591086891No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT119440.19965898833205176No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA103050.17226104108856274No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA96510.16132860820433953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)