FastQCFastQC Report
Tue 6 Sep 2022
N57_S191_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN57_S191_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5465096
Sequences flagged as poor quality0
Sequence length20-86
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC72660.1329528337654087No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG72610.1328613440642214No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC65580.11999789207728465No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT61890.11324595212966067No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA55300.10118760951317231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)