FastQCFastQC Report
Tue 6 Sep 2022
N55_S190_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN55_S190_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753992
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC89190.23758708063309672No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG83990.2237351597978898No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC75640.20149217153366336No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT68750.18313837642701422No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA63460.16904671080812106No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA55360.14746968027635649No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC49880.1328718867807923No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG43870.11686226289240892No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA40760.10857774870058329No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA40090.10679298197758547No Hit
GCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACCCAACCTCTC39470.10514140680108001No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG37930.10103910716911492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)