FastQCFastQC Report
Tue 6 Sep 2022
N54_S193_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN54_S193_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5875424
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG262250.44635076549369035No Hit
CCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGG201860.34356669408029106No Hit
GGCCCCGTAATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCAT182410.3104627002238477No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG139450.2373445729193331No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC132120.2248688775482416No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC120840.20567026311632997No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC115140.19596883561084272No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT112610.1916627634022668No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG109550.1864546286361631No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG107890.18362930062579313No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG98400.1674772748315696No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA98140.16703475357693334No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA93620.15934169176556448No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT89660.15260175265648912No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA88200.15011682561122397No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG86450.14713831716655681No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT85010.14468743021780214No Hit
AACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTC84270.14342794664691433No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC82120.13976863627203756No Hit
TGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTC75800.12901196577472537No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT69900.11897013730413329No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA68500.11658733054839958No Hit
GTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCT66680.11348968176594573No Hit
AGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTT65470.11143025592706161No Hit
AGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACATCCAAGG64200.10926870979864603No Hit
CGGGGGCATTCGTATGGCGGGGTTAGAGGTGAAATTCTTGGATCCTCGCC61900.10535409869994064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)