FastQCFastQC Report
Tue 6 Sep 2022
N50_S187_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN50_S187_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5655005
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC101350.17922176903468698No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC99190.1754021437646828No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG89700.15862054940711812No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC87310.1543942047796598No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG83810.1482049971662271No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG76270.13487167562186064No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT74860.13237830912616347No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA72260.12778061204189917No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT68010.12026514565415945No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA66050.11679918939063715No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA63960.11310334827290161No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA62490.11050388107525988No Hit
GGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGTCCTCTTG61990.10961970855905522No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG60950.10778062972534949No Hit
GAACTGTGGTGTTTGGGACGTCTGTTGTGGTCGTGTGCCGGAGCCCAAGT58920.10419088930955851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)