FastQCFastQC Report
Tue 6 Sep 2022
N49_S185_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN49_S185_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4535410
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC70490.15542145032091917No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG66950.14761620228380676No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC63230.13941407722785812No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT56940.12554543029185897No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC54770.12076085734255557No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA51810.11423443525502656No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG50080.11042000612954507No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA46030.10149027320573002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)