FastQCFastQC Report
Tue 6 Sep 2022
N48_S194_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN48_S194_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4087487
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC87700.21455725730748504No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGG82390.20156639030289272No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTC79280.19395780341319738No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATT70070.1714256216594695No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAA68780.1682696483193708No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAA59200.14483226490995568No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC51480.12594535468858983No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG43140.10554162007120757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)