FastQCFastQC Report
Tue 6 Sep 2022
N42_S167_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameN42_S167_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4781657
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG62880.13150253144464355No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC57140.11949832453477947No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA50320.10523548635964478No Hit
TGTGTTAAAGATACTCACTAGACCAGGATATTATAAGTGAAAAATTCGGC49680.10389703820244739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)