FastQCFastQC Report
Tue 6 Sep 2022
M47_S162_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM47_S162_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5604371
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC90590.16164169003087056No Hit
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG73560.1312546938809012No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC66550.1187465997522291No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG62960.11234088535537709No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC62290.11114538991083925No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA59510.10618497597678668No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG59330.10586379809616457No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT57740.10302672681733596No Hit
GCTGGCGGTCCGCCTCGTTGCGGTCACTGCCTGTCCTGACCCAACCTCTC56310.10047514698794922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)