FastQCFastQC Report
Tue 6 Sep 2022
M46_S141_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM46_S141_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5089908
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGTG101940.20027866908399913No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGAC86780.1704942407603438No Hit
TGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGC84820.16664348353644115No Hit
GAAGAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTT81150.1594331371018887No Hit
TAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTC78480.15418746272034778No Hit
GAGCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTG78340.15391240863292616No Hit
GTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGG76510.150317058775915No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATGGCGGGGTTAGA70980.13945242232276106No Hit
GCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACG66040.1297469423808839No Hit
GTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATT63860.12546395730531867No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCACA63260.12428515407351173No Hit
GCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAA60970.11978605507211525No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACC57520.11300793648922536No Hit
GCGCTTTTATTAGATCAAAACCGATCGGTCGCAAGGCCGTCCTTTTTGGT52680.10349892375264935No Hit
GGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAA51480.10114131728903548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)