FastQCFastQC Report
Tue 12 Dec 2023
T135G_S145_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameT135G_S145_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488023
Total Bases251.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCTGGGAAAAAGAATAAGAGTGATGATGTATCCATCAAGAGTATAA41430.16651775325228102No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGATCGGAAGAGCACACG35440.14244241311274053No Hit
ACCAGGGAAATGTGGAAGGGCTGATGGCTACATCCTAGAAGGAAAAGAAC30200.12138151455995383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)