FastQCFastQC Report
Thu 14 Dec 2023
G8-T051G_S162_L099_R1_cmb.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG8-T051G_S162_L099_R1_cmb.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775980
Total Bases55.3 Mbp
Sequences flagged as poor quality0
Sequence length20-95
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGA39630.510709038892755No Hit
AAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCT36250.46715121523750613No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG29670.3823552153406016No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG21310.274620479909276No Hit
TTTGCAGTTATATTTATTGCGGAGTACTCTAGTATTCTTCTCAGAAGGGT18150.233897780870641No Hit
CTCTTATACGATTTTGATGGGAGATCAGTG15800.20361349519317506No Hit
AGTTTTTGGCTCTTATACGATTTTGATGGGAGATCAGTG14940.1925307353282301No Hit
TCTGTTCGCCCTTTAAAATCTAACATGAGCTG11960.15412768370318822No Hit
AATGGTTATGGGGTTAAGAGTTGCGTTTGTCTGCATTGTGTCTTTTTTGT11230.1447202247480605No Hit
CTAGCTACTCTAGGGATAACAGCGCAATTTCTCCCGAAAGATGGTATTGG11120.14330266243975362No Hit
ACTCTTTGACTAATGGTGAGGTTAGTCCGACTATGGCGTGGGGAGTTTTC10870.14008092992087426No Hit
AGTTTTCCAGTCAACGGCACTTTGGTTTTGAGGCTTGCATTTGGTACTGA10450.13466841928915693No Hit
ATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCT9610.12384339802572232No Hit
TCCGCTGTGTCTTTTGGGTCTCTAATATATTTTAAGTTCCTTCTATGAGA9240.11907523389778087No Hit
TGGCTAGCTACTCTAGGGATAACAGCGCAATTTCTCCCGAAAGATGGTAT8860.11417820046908427No Hit
CCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGT8610.1109564679502049No Hit
ACGTGTGCTCTTCCGATCTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.1109564679502049No Hit
ATGGTATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCT8370.10786360473208072No Hit
TAAAATTTTTTGGGTTAAGCTGAAAAATACTAAAATTGATGTT8360.10773473543132556No Hit
CAGAGATTTAATTAAACTAAAGAATAGCGGCTAGATACTATTCGCGCTTT8320.10721925822830486No Hit
ATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTAATGGTGGGTCT8290.10683265032603934No Hit
TTTTTCAAGACAAGTTTAAGAATTTAATTTTCCCG8270.10657491172452899No Hit
TATTTCCTAACTTTATATGTGTGGCTAGCTACTCTAGGGATAACAGCGCA8260.10644604242377381No Hit
TCTCTAATATATTTTAAGTTCCTTCTATGAGAGGCTCTAGTAAGCCAGCG8230.10605943452150829No Hit
TCCTAACTTTATATGTGTGGCTAGCTACTCTAGGGATAACAGCGCAATTT8230.10605943452150829No Hit
CAGTGTCTTTCAGTCATCTATTTCTCTAGGTTGTTATTAAACCCCCTTTC7890.10167787829583237No Hit
ATCCTGGGTGTTAAAGGATAACCCACCTACATTTGAGCCTTGTCTTATTA7760.10000257738601509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)