FastQCFastQC Report
Thu 14 Dec 2023
F4-T007FX_S225_L099_R1_cmb.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameF4-T007FX_S225_L099_R1_cmb.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075901
Total Bases138 Mbp
Sequences flagged as poor quality0
Sequence length20-95
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGT31000.14933274756358805No Hit
AAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCT29080.14008375158545613No Hit
ATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGA28130.13550742545044298No Hit
ATGAACCAAACGTTCGGTTAAGGTGCCAAACACGGACGCTCATCAGATAC27710.1334842075802266No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG22580.10877204645115543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)