FastQCFastQC Report
Thu 14 Dec 2023
E4-T130F_S128_L099_R1_cmb.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameE4-T130F_S128_L099_R1_cmb.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361461
Total Bases156 Mbp
Sequences flagged as poor quality0
Sequence length20-95
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGAACCAAACGTTCGGTTAAGGTGCCAAACACGGACGCTCATCAGATAC25530.10811103803958651No Hit
CCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGT24490.10370698478611333No Hit
AAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCT23780.10070037150729994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)