@SQ SN:zz LN:30 @CO All MD and/or NM should differ to the stored values a1 0 zz 6 1 10M * 0 0 AAAAATTTTT * MD:Z:9 NM:i:0 co:Z:MD incorrect fields a2 0 zz 6 1 10M * 0 0 AAAAGGTTTT * MD:Z:4A0A4 NM:i:2 a3 0 zz 6 1 10M * 0 0 GAAAATTTTG * MD:Z:0G8T0 NM:i:2 i1 0 zz 6 1 5M1I5M * 0 0 AAAAAGTTTTT * MD:Z:11 NM:i:1 i2 0 zz 6 1 5M3I5M * 0 0 AAAAAGGGTTTTT * MD:Z:1A1 NM:i:3 i3 0 zz 6 1 10M2I * 0 0 AAAAATTTTTCC * MD:Z:12 NM:i:2 d1 0 zz 6 1 5M10D5M * 0 0 AAAAACCCCC * MD:Z:5^CTTTTTTTTT5 NM:i:10 d2 0 zz 6 1 5M10N5M * 0 0 AAAAACCCCC * MD:Z:9 NM:i:0 sid 0 zz 6 1 1S4M10D5I4M1S * 0 0 AAAAAGGGGGCCCCC * MD:Z:4^TTTTTTTTT0T3 NM:i:16 A1 0 zz 6 1 10M * 0 0 AAAAATTTTT * MD:Z:10 NM:i:1 co:Z:NM incorrect fields A2 0 zz 6 1 10M * 0 0 AAAAGGTTTT * MD:Z:4A0T4 NM:i:0 A3 0 zz 6 1 10M * 0 0 GAAAATTTTG * MD:Z:0A8T0 NM:i:0 I1 0 zz 6 1 5M1I5M * 0 0 AAAAAGTTTTT * MD:Z:10 NM:i:0 I2 0 zz 6 1 5M3I5M * 0 0 AAAAAGGGTTTTT * MD:Z:10 NM:i:0 I3 0 zz 6 1 10M2I * 0 0 AAAAATTTTTCC * MD:Z:10 NM:i:0 D1 0 zz 6 1 5M10D5M * 0 0 AAAAACCCCC * MD:Z:5^TTTTTTTTTT5 NM:i:11 D2 0 zz 6 1 5M10N5M * 0 0 AAAAACCCCC * MD:Z:10 NM:i:1 SID 0 zz 6 1 1S4M10D5I4M1S * 0 0 AAAAAGGGGGCCCCC * MD:Z:4^ATTTTTTTTT0T3 NM:i:1