@CO xx has been encoded using the SAM spec; leftmost to rightmost @CO yy has been encoded using bwa/picard methods; 5' to 3' @CO @CO 00000000011111111112 @CO 12345678901234567890 @CO AAAAAAAAAATTTTTTTTTT @CO 1>>>> <<<<2 x1 @CO @CO 1>>>> x2 @CO <<<<2 @CO @CO 1>>>> x3 (7..15 vs 10..11) @CO <<<<2 @CO @CO <<<<2 1>>>> x4 (1..20 vs 16..5) @CO @SQ SN:xx LN:20 @SQ SN:yy LN:20 x1 99 xx 1 1 5M = 16 20 AAAAA ***** x1 147 xx 16 1 5M = 1 -20 TTTTT ***** x2 99 xx 7 1 5M = 10 8 AAAAT ***** x2 147 xx 10 1 5M = 7 -8 ATTTT ***** x3 147 xx 7 1 5M = 10 8 AAAAT ***** x3 99 xx 10 1 5M = 7 -8 ATTTT ***** x4 147 xx 1 1 5M = 16 20 AAAAA ***** x4 99 xx 16 1 5M = 1 -20 TTTTT ***** y1 99 yy 1 1 5M = 16 20 AAAAA ***** y1 147 yy 16 1 5M = 1 -20 TTTTT ***** y2 99 yy 7 1 5M = 10 8 AAAAT ***** y2 147 yy 10 1 5M = 7 -8 ATTTT ***** y3 147 yy 7 1 5M = 10 -2 AAAAT ***** y3 99 yy 10 1 5M = 7 2 ATTTT ***** y4 147 yy 1 1 5M = 16 10 AAAAA ***** y4 99 yy 16 1 5M = 1 -10 TTTTT *****