FastQCFastQC Report
Tue 25 Apr 2023
JV173_UniCOI_Tornabene_S043451.1.R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJV173_UniCOI_Tornabene_S043451.1.R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10886
Sequences flagged as poor quality0
Sequence length250
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGACTTCGGGATGGCCAAAAAATCAAAACAGGTGTTGGTATAAGATGGG3262.9946720558515527No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAACAGGTGTTGGTATAAGATGGG3082.829322065037663No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAACAGGTGTTGGTATAAGATGGG3032.783391512033805No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAACAGGTGTTGGTATAAGATGGG2972.7282748484291752No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAACAGGTGTTGGTATAAGATGGG2942.7007165166268603No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAACAGGTGTTGGTATAAGATGGG2922.6823442954253167No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAACAGGTGTTGGTATAAGATGGG2892.6547859636230022No Hit
TAGACCTCGGGATGGCCAAAAAATCAAAACAGGTGTTGGTATAAGATGGG2752.526180415212199No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAACAGGTGTTGGTATAAGATGGG2662.4435054198052546No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAACAGGTGTTGGTATAAGATGGG2652.434319309204483No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAACAGGTGTTGGTATAAGATGGG2622.406760977402168No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAACAGGTGTTGGTATAAGATGGG2592.379202645599853No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAACAGGTGTTGGTATAAGATGGG2422.223038765386735No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAACAGGTGTTGGTATAAGATGGG2222.0393165533713025No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAACAGGTGTTGGTATAAGATGGG2212.0301304427705307No Hit
TAGACTTCGGGATGGCCGAAAAACCAAAACAGGTGTTGGTATAAGATGGG2192.0117582215689875No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAACAGGTGTTGGTATAAGATGGG2151.975013779165901No Hit
TAGACTTCGGGATGGCCAAAAAACCAAAACAGGTGTTGGTATAAGATGGG2121.9474554473635861No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAACAGGTGTTGGTATAAGATGGG2001.8372221201543266No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAACAGGTGTTGGTATAAGATGGG1991.828036009553555No Hit
TAGACCTCGGGATGGCCGAAGAACCAAAACAGGTGTTGGTATAAGATGGG1971.8096637883520117No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAACAGGTGTTGGTATAAGATGGG1951.7912915671504683No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAACAGGTGTTGGTATAAGATGGG1921.7637332353481534No Hit
TAGACCTCGGGGTGGCCGAAAAACCAAAACAGGTGTTGGTATAAGATGGG1851.6994304611427522No Hit
TAGACCTCGGGGTGGCCAAAGAACCAAAACAGGTGTTGGTATAAGATGGG1771.625941576336579No Hit
TAGACCTCGGGATGGCCGAAAAACCAAAACAGGTGTTGGTATAAGATGGG1771.625941576336579No Hit
TAGACTTCGGGGTGGCCAAAAAACCAAAACAGGTGTTGGTATAAGATGGG1761.6167554657358074No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAACAGGTGTTGGTATAAGATGGG1721.5800110233327211No Hit
TAGACTTCGGGATGGCCAAAGAACCAAAACAGGTGTTGGTATAAGATGGG1671.5340804703288629No Hit
TAGACCTCGGGATGGCCAAAAAACCAAAACAGGTGTTGGTATAAGATGGG1581.451405474921918No Hit
TAGACCTCGGGATGGCCAAAGAACCAAAACAGGTGTTGGTATAAGATGGG1581.451405474921918No Hit
TAGACCTCGGGGTGGCCAAAAAACCAAAACAGGTGTTGGTATAAGATGGG1231.129891603894911No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAATAGGTGCTGGTACAGAATAGG260.23883887562006245No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAATAGGTGCTGGTACAGAATAGG220.20209443321697593No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG210.1929083226162043No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAATAGGTGCTGGTACAGAATAGG210.1929083226162043No Hit
TAGACTTCGGGATGGCCAAAAAATCAGAATAGGTGCTGGTACAGAATAGG200.18372221201543268No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAATAGGTGCTGGTACAGAATAGG200.18372221201543268No Hit
TAGACTTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG200.18372221201543268No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAATAGGTGCTGGTACAGAATAGG200.18372221201543268No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAATAGGTGCTGGTACAGAATAGG190.17453610141466103No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAATAGGTGCTGGTACAGAATAGG180.1653499908138894No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG180.1653499908138894No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAATAGGTGCTGGTACAGAATAGG180.1653499908138894No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAACAGGTGTTGGTATAAGATGGG180.1653499908138894No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAATAGGTGCTGGTACAGAATAGG170.15616388021311775No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAATAGGTGCTGGTACAGAATAGG170.15616388021311775No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAATAGGTGCTGGTACAGAATAGG170.15616388021311775No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAACAGGTGTTGGTATAAGATGGG170.15616388021311775No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG170.15616388021311775No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG160.14697776961234615No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAACAGGTGTTGGTATAAGATGGG160.14697776961234615No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAATAGGTGCTGGTACAGAATAGG160.14697776961234615No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAATAGGTGCTGGTACAGAATAGG150.1377916590115745No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAACAGGTGTTGGTATAAGATGGG150.1377916590115745No Hit
TAGACTTCGGGATGGCCAAAAAATCAGAACAGGTGTTGGTATAAGATGGG150.1377916590115745No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAATAGGTGCTGGTACAGAATAGG140.12860554841080288No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG140.12860554841080288No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAATAGGTGCTGGTACAGAATAGG140.12860554841080288No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG140.12860554841080288No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG140.12860554841080288No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAATAGGTGCTGGTACAGAATAGG140.12860554841080288No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG140.12860554841080288No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG130.11941943781003123No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAACAGGTGTTGGTATAAGATGGG130.11941943781003123No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAATAGGTGCTGGTACAGAATAGG130.11941943781003123No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAATAGGTGCTGGTACAGAATAGG130.11941943781003123No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAATAGGTGCTGGTACAGAATAGG130.11941943781003123No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAATAGGTGCTGGTACAGAATAGG130.11941943781003123No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAACAGGTGTTGGTATAAGATGGG130.11941943781003123No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAATAGGTGCTGGTACAGAATAGG130.11941943781003123No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG130.11941943781003123No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAATAGGTGCTGGTACAGAATAGG120.11023332720925959No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAATAGGTGCTGGTACAGAATAGG120.11023332720925959No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG120.11023332720925959No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG120.11023332720925959No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAACAGGTGTTGGTATAAGATGGG120.11023332720925959No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG120.11023332720925959No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAATAGGTGCTGGTACAGAATAGG120.11023332720925959No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAACAGGTGTTGGTATAAGATGGG120.11023332720925959No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG120.11023332720925959No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAATAGGTGCTGGTACAGAATAGG120.11023332720925959No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAATAGGTGCTGGTACAGAATAGG120.11023332720925959No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAACAGGTGTTGGTATAAGATGGG120.11023332720925959No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAACAGGTGTTGGTATAAGATGGG110.10104721660848796No Hit
TAGACTTCGGGCTGGCCGAAAAATCAAAACAGGTGTTGGTATAAGATGGG110.10104721660848796No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG110.10104721660848796No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG110.10104721660848796No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG110.10104721660848796No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAACAGGTGTTGGTATAAGATGGG110.10104721660848796No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG110.10104721660848796No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAATAGGTGCTGGTACAGAATAGG110.10104721660848796No Hit

[OK]Adapter Content

Adapter graph