FastQCFastQC Report
Tue 25 Apr 2023
JV173_UniCOI_Tornabene_S043450.1.R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJV173_UniCOI_Tornabene_S043450.1.R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10891
Sequences flagged as poor quality0
Sequence length250
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGACTTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1901.744559728215958No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1891.7353778349095583No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1751.6068313286199614No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1741.5976494353135615No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1681.542558075475163No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1601.4691029290239648No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1581.450739142411165No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1561.4323753557983656No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1551.4231934624919658No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1541.4140115691855661No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1491.3681021026535671No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1441.3221926361215683No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1391.2762831695895693No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1361.24873748967037No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1361.24873748967037No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1341.2303737030575705No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1261.1569185566063722No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1201.1018271967679736No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1201.1018271967679736No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG1191.0926453034615737No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG1181.0834634101551741No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG1171.0742815168487743No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1161.0650996235423744No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG1111.0191901570103756No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTATAGGATTGG1070.9824625837847764No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1050.9640987971719769No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1010.9273712239463777No Hit
TAGACTTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTATAGGATTGG980.8998255440271784No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTATAGGATTGG980.8998255440271784No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG960.8814617574143788No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTATAGGATTGG950.872279864107979No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTATAGGATTGG940.8630979708015792No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTATAGGATTGG940.8630979708015792No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTATAGGATTGG900.8263703975759802No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG900.8263703975759802No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTATAGGATTGG890.8171885042695803No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG890.8171885042695803No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTATAGGATTGG880.8080066109631806No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG870.7988247176567808No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTATAGGATTGG860.7896428243503811No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG850.7804609310439813No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG830.7620971444311817No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTATAGGATTGG830.7620971444311817No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTATAGGATTGG800.7345514645119824No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTATAGGATTGG770.7070057845927831No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTATAGGATTGG770.7070057845927831No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTATAGGATTGG760.6978238912863832No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTATAGGATTGG690.6335506381415847No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTATAGGATTGG680.624368744835185No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTATAGGATTGG650.5968230649159857No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTATAGGATTGG640.5876411716095858No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAGAGAATGGG640.5876411716095858No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAGAGAATGGG620.5692773849967864No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAGAGAATGGG600.5509135983839868No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTATAGGATTGG600.5509135983839868No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAGAGAATGGG580.5325498117711872No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTATAGGATTGG580.5325498117711872No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTATAGGATTGG580.5325498117711872No Hit
TAGACTTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAGAGAATGGG570.5233679184647874No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAGAGAATGGG570.5233679184647874No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTATAGGATTGG570.5233679184647874No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAGAGAATGGG570.5233679184647874No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTATAGGATTGG550.5050041318519879No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTATAGGATTGG550.5050041318519879No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAGAGAATGGG550.5050041318519879No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAGAGAATGGG550.5050041318519879No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTATAGGATTGG540.49582223854558805No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTATAGGATTGG510.46827655862638873No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAGAGAATGGG500.45909466531998894No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTATAGGATTGG490.4499127720135892No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTATAGGATTGG490.4499127720135892No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAGAGAATGGG490.4499127720135892No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAGAGAATGGG490.4499127720135892No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTATAGGATTGG470.4315489854007896No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTATAGGATTGG470.4315489854007896No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAGAGAATGGG460.4223670920943899No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAGAGAATGGG440.4040033054815903No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAGAGAATGGG440.4040033054815903No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTATAGGATTGG440.4040033054815903No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAGAGAATGGG420.3856395188687908No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAGAGAATGGG420.3856395188687908No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAGAGAATGGG420.3856395188687908No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAGAGAATGGG400.3672757322559912No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAGAGAATGGG400.3672757322559912No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGATAGAGAATAGG390.3580938389495914No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAGAGAATGGG380.3489119456431916No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAGAGAATGGG370.3397300523367919No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAGAGAATGGG370.3397300523367919No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGATAGAGAATAGG350.3213662657239923No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGATAGAGAATAGG340.3121843724175925No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAGAGAATGGG330.3030024791111927No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGTTGATAGAGAATAGG330.3030024791111927No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAGAGAATGGG320.2938205858047929No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAGAGAATGGG320.2938205858047929No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAGAGAATGGG320.2938205858047929No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGATAGAGAATAGG310.2846386924983932No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAGAGAATGGG300.2754567991919934No Hit
TAGACTTCGGGATGGCCAAAAAATCAAAATAAATGTTGATAGAGAATAGG300.2754567991919934No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAGAGAATGGG300.2754567991919934No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAGAGAATGGG290.2662749058855936No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGATAGAGAATAGG280.2570930125791938No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAGAGAATGGG270.24791111927279402No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAATAAATGTTGATAGAGAATAGG260.2387292259663943No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGATAGAGAATAGG260.2387292259663943No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAGAGAATGGG250.22954733265999447No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAATGTTGATAGAGAATAGG250.22954733265999447No Hit
TAGACCTCGGGATGGCCAAAAAATCAAAATAAATGTTGATAGAGAATAGG240.2203654393535947No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAATAAATGTTGATAGAGAATAGG230.21118354604719494No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGATAGAGAATAGG220.20200165274079515No Hit
TAGACCTCGGGGTGGCCAAAGAACCAAAATAAATGTTGATAGAGAATAGG220.20200165274079515No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAATGTTGATAGAGAATAGG220.20200165274079515No Hit
TAGACTTCGGGGTGGCCAAAAAACCAAAATAAATGTTGATAGAGAATAGG210.1928197594343954No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGATAGAGAATAGG210.1928197594343954No Hit
TAGACCTCGGGATGGCCGAAGAACCAAAATAAATGTTGATAGAGAATAGG210.1928197594343954No Hit
TAGACCTCGGGATGGCCGAAAAACCAAAATAAATGTTGATAGAGAATAGG200.1836378661279956No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAATAAATGTTGATAGAGAATAGG190.1744559728215958No Hit
TAGACTTCGGGATGGCCGAAAAACCAAAATAAATGTTGATAGAGAATAGG180.16527407951519604No Hit
TAGACTTCGGGATGGCCAAAGAACCAAAATAAATGTTGATAGAGAATAGG180.16527407951519604No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGATAGAGAATAGG180.16527407951519604No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAATGTTGATAGAGAATAGG170.15609218620879625No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAATAAATGTTGATAGAGAATAGG170.15609218620879625No Hit
TAGACCTCGGGATGGCCAAAGAACCAAAATAAATGTTGATAGAGAATAGG140.1285465062895969No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAATAAATGTTGATAGAGAATAGG140.1285465062895969No Hit
TAGACTTCGGGATGGCCAAAAAACCAAAATAAATGTTGATAGAGAATAGG140.1285465062895969No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAATAAATGTTGATAGAGAATAGG130.11936461298319714No Hit
TAGACCTCGGGGTGGCCGAAAAACCAAAATAAATGTTGATAGAGAATAGG120.11018271967679735No Hit
TAGACCTCGGGATGGCCAAAAAACCAAAATAAATGTTGATAGAGAATAGG110.10100082637039758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)