FastQCFastQC Report
Tue 25 Apr 2023
JV173_UniCOI_Tornabene_S043449.1.R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJV173_UniCOI_Tornabene_S043449.1.R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9692
Sequences flagged as poor quality0
Sequence length250
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGACTTCGGGATGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATCGG1741.7952950887329757No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1591.6405282707387538No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1581.6302104828724722No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATCGG1561.609574907139909No Hit
TAGACTTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1561.609574907139909No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1531.5786215435410649No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATCGG1521.5683037556747832No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATCGG1511.5579859678085017No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATCGG1481.5270326042096576No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1421.4651258770119686No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1401.4444903012794057No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1331.3722657862154355No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATCGG1321.361947998349154No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATCGG1311.3516302104828726No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATCGG1301.3413124226165911No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1291.3309946347503094No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATCGG1251.2897234832851836No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATCGG1241.2794056954189021No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATCGG1231.2690879075526207No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1211.2484523318200578No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATCGG1181.2174989682212134No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATCGG1181.2174989682212134No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1161.1968633924886503No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1141.1762278167560873No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1131.165910028889806No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATCGG1131.165910028889806No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1121.1555922410235244No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1121.1555922410235244No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG1111.1452744531572432No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATCGG1111.1452744531572432No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATCGG1101.1349566652909615No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1101.1349566652909615No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1081.1143210895583986No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG1071.1040033016921171No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATCGG1051.0833677259595542No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATCGG1041.0730499380932728No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1041.0730499380932728No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATCGG1011.0420965744944284No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATCGG1011.0420965744944284No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG1001.031778786628147No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATCGG991.0214609987618655No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATCGG981.011143210895584No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG981.011143210895584No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATCGG971.0008254230293026No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATCGG960.990507635163021No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG910.9389186958316137No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATCGG900.9286009079653321No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATCGG880.9079653322327692No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG860.8873297565002063No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG860.8873297565002063No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATCGG840.8666941807676434No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG840.8666941807676434No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATCGG830.856376392901362No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG800.8254230293025175No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG790.8151052414362361No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATCGG750.7738340899711103No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG730.7531985142385473No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG710.7325629385059843No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG710.7325629385059843No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG690.7119273627734214No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATCGG680.7016095749071399No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG680.7016095749071399No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATCGG640.6603384234420141No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATCGG560.5777961205117622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)