FastQCFastQC Report
Tue 25 Apr 2023
JV173_UniCOI_Tornabene_S043448.1.R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJV173_UniCOI_Tornabene_S043448.1.R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16382
Sequences flagged as poor quality0
Sequence length250
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGACTTCGGGATGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATTGG4933.0094005615919914No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATTGG4782.9178366499816875No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATTGG4682.856794042241485No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATTGG4612.814064216823343No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATTGG4502.74691734830912No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATTGG4492.7408130875351No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATTGG4252.5943108289586134No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATTGG4192.557685264314492No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATTGG4112.5088511781223297No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATTGG4102.5027469173483095No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATTGG4092.496642656574289No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATTGG4022.4539128311561473No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATTGG3732.276889268709559No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATTGG3632.2158466609693566No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATTGG3492.130387010133073No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATTGG3452.105969967036992No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATTGG3282.0021975338786473No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATTGG3282.0021975338786473No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATTGG3241.9777804907825662No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATTGG3131.9106336222683433No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATTGG3071.8740080576242217No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATTGG2991.8251739714320596No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATTGG2991.8251739714320596No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATTGG2941.7946526675619583No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATTGG2921.7824441460139178No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATTGG2811.7152972774996949No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATTGG2801.7091930167256746No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATTGG2641.6115248443413503No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATTGG2481.5138566719570261No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATTGG2331.4222927603467221No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATTGG2291.397875717250641No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATTGG2081.2696862409962155No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG650.3967769503113173No Hit
TAGACTTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG600.366255646441216No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG570.3479428641191552No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG560.34183860334513494No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG540.3296300817970944No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG520.3174215602490539No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG490.2991087779269931No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG470.2869002563789525No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG450.27469173483091197No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG450.27469173483091197No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG440.2685874740568917No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG420.2563789525088512No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG420.2563789525088512No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG420.2563789525088512No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG410.2502746917348309No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG400.24417043096081065No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG400.24417043096081065No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG390.23806617018679038No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG380.23196190941277012No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG380.23196190941277012No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG380.23196190941277012No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG370.22585764863874985No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG370.22585764863874985No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG350.21364912709070932No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG290.17702356244658773No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG290.17702356244658773No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG270.1648150408985472No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG270.1648150408985472No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG260.15871078012452694No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG240.1465022585764864No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG230.14039799780246612No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG200.12208521548040532No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAACAGGTGTTGATTGGATCACCC190.11598095470638506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)