FastQCFastQC Report
Tue 25 Apr 2023
JV173_UniCOI_Tornabene_S043448.1.R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJV173_UniCOI_Tornabene_S043448.1.R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16382
Sequences flagged as poor quality0
Sequence length250
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACTGGTTGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC2481.5138566719570261No Hit
GGTACTGGTTGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC2281.3917714564766208No Hit
GGTACTGGTTGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1931.1781223293859115No Hit
GGTACAGGTTGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1911.1659138078378708No Hit
GGTACTGGTTGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1871.1414967647417897No Hit
GGTACTGGTTGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1871.1414967647417897No Hit
GGTACTGGATGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1871.1414967647417897No Hit
GGAACTGGTTGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1861.1353925039677695No Hit
GGTACTGGTTGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1851.1292882431937492No Hit
GGTACTGGTTGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1731.056037113905506No Hit
GGTACTGGATGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1651.007203027713344No Hit
GGTACTGGTTGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1550.9461604199731413No Hit
GGTACTGGTTGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1500.9156391161030399No Hit
GGTACAGGTTGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1490.9095348553290197No Hit
GGAACTGGTTGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1450.8851178122329386No Hit
GGAACTGGTTGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1420.8668050299108778No Hit
GGTACAGGTTGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1380.8423879868147968No Hit
GGTACAGGTTGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1360.8301794652667562No Hit
GGTACAGGTTGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1360.8301794652667562No Hit
GGTACTGGTTGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1340.8179709437187157No Hit
GGTACAGGATGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1320.8057624221706752No Hit
GGTACTGGATGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1290.7874496398486144No Hit
GGTACTGGTTGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1280.7813453790745941No Hit
GGAACTGGTTGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1270.7752411183005739No Hit
GGAACTGGTTGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1270.7752411183005739No Hit
GGTACTGGTTGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC1270.7752411183005739No Hit
GGAACTGGTTGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1250.7630325967525333No Hit
GGAACAGGTTGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1240.7569283359785131No Hit
GGTACTGGTTGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1230.7508240752044928No Hit
GGTACAGGTTGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1230.7508240752044928No Hit
GGAACTGGATGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1230.7508240752044928No Hit
GGTACTGGATGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1220.7447198144304725No Hit
GGTACTGGATGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1200.732511292882432No Hit
GGTACAGGTTGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1190.7264070321084117No Hit
GGTACAGGTTGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1180.7203027713343915No Hit
GGTACTGGATGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1180.7203027713343915No Hit
GGTACTGGATGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1170.7141985105603712No Hit
GGTACTGGATGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1160.7080942497863509No Hit
GGAACTGGTTGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1160.7080942497863509No Hit
GGTACAGGATGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1140.6958857282383104No Hit
GGAACAGGTTGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1120.6836772066902699No Hit
GGAACTGGATGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1120.6836772066902699No Hit
GGTACTGGATGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1110.6775729459162496No Hit
GGAACTGGATGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1090.6653644243682091No Hit
GGTACAGGATGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1090.6653644243682091No Hit
GGTACTGGTTGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1080.6592601635941888No Hit
GGAACTGGTTGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1060.6470516420461483No Hit
GGAACTGGTTGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1060.6470516420461483No Hit
GGTACAGGATGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1050.640947381272128No Hit
GGTACAGGTTGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1030.6287388597240875No Hit
GGTACAGGTTGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1020.6226345989500672No Hit
GGTACAGGATGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1020.6226345989500672No Hit
GGAACTGGATGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC990.6043218166280064No Hit
GGTACTGGATGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC990.6043218166280064No Hit
GGAACTGGTTGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC970.5921132950799659No Hit
GGTACAGGTTGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC960.5860090343059456No Hit
GGTACAGGATGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC950.5799047735319253No Hit
GGAACTGGTTGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC920.5615919912098645No Hit
GGAACAGGTTGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC910.5554877304358442No Hit
GGAACTGGATGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC910.5554877304358442No Hit
GGAACTGGTTGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC910.5554877304358442No Hit
GGAACAGGTTGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC900.5493834696618239No Hit
GGAACTGGATGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC890.5432792088878037No Hit
GGAACAGGTTGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC880.5371749481137834No Hit
GGAACTGGTTGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC880.5371749481137834No Hit
GGTACAGGATGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC880.5371749481137834No Hit
GGAACAGGATGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC870.5310706873397631No Hit
GGTACTGGTTGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC860.5249664265657429No Hit
GGTACAGGATGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC840.5127579050177024No Hit
GGTACTGGATGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC840.5127579050177024No Hit
GGTACTGGATGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC830.5066536442436821No Hit
GGTACTGGATGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC820.5005493834696618No Hit
GGTACAGGTTGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC820.5005493834696618No Hit
GGTACAGGTTGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC820.5005493834696618No Hit
GGTACAGGATGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC810.49444512269564156No Hit
GGTACTGGTTGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC810.49444512269564156No Hit
GGAACTGGATGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC800.4883408619216213No Hit
GGTACAGGTTGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC800.4883408619216213No Hit
GGTACAGGATGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC790.48223660114760103No Hit
GGAACTGGTTGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC780.47613234037358076No Hit
GGAACAGGTTGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC780.47613234037358076No Hit
GGAACTGGATGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC760.46392381882554024No Hit
GGAACAGGTTGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC740.4517152972774997No Hit
GGAACAGGTTGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC740.4517152972774997No Hit
GGAACAGGATGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC730.44561103650347944No Hit
GGTACTGGATGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC730.44561103650347944No Hit
GGTACAGGATGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC730.44561103650347944No Hit
GGAACAGGTTGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC720.4395067757294592No Hit
GGTACTGGATGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC720.4395067757294592No Hit
GGAACTGGATGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC720.4395067757294592No Hit
GGAACAGGATGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC700.42729825418141865No Hit
GGAACTGGTTGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC700.42729825418141865No Hit
GGAACTGGATGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC690.4211939934073984No Hit
GGAACTGGATGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC670.40898547185935785No Hit
GGAACAGGATGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC660.4028812110853376No Hit
GGAACAGGATGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC650.3967769503113173No Hit
GGTACAGGTTGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC630.3845684287632768No Hit
GGTACAGGATGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC610.37235990721523626No Hit
GGAACTGGATGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC600.366255646441216No Hit
GGAACTGGTTGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC600.366255646441216No Hit
GGAACAGGATGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC600.366255646441216No Hit
GGTACAGGATGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC590.36015138566719573No Hit
GGTACAGGTTGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC590.36015138566719573No Hit
GGAACAGGATGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC590.36015138566719573No Hit
GGAACTGGATGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC590.36015138566719573No Hit
GGAACAGGTTGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC580.35404712489317547No Hit
GGAACAGGATGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC580.35404712489317547No Hit
GGAACAGGATGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC570.3479428641191552No Hit
GGTACAGGATGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC560.34183860334513494No Hit
GGAACAGGTTGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC560.34183860334513494No Hit
GGAACAGGTTGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC520.3174215602490539No Hit
GGAACTGGATGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC520.3174215602490539No Hit
GGAACAGGTTGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC490.2991087779269931No Hit
GGTACAGGATGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC490.2991087779269931No Hit
GGAACAGGTTGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC470.2869002563789525No Hit
GGTACTGGATGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC470.2869002563789525No Hit
GGAACTGGATGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC470.2869002563789525No Hit
GGAACAGGATGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC470.2869002563789525No Hit
GGAACAGGTTGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC440.2685874740568917No Hit
GGAACAGGATGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC440.2685874740568917No Hit
GGAACTGGATGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC440.2685874740568917No Hit
GGAACAGGATGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC440.2685874740568917No Hit
GGAACAGGTTGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC430.26248321328287144No Hit
GGAACAGGATGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC410.2502746917348309No Hit
GGTACAGGATGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC410.2502746917348309No Hit
GGAACAGGATGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC360.2197533878647296No Hit
GGAACAGGATGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC350.21364912709070932No Hit
GGAACAGGATGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC250.15260651935050668No Hit
GGTACTGGTTGAACAGTTTATCCTCCCTTAGCGGGCAATCTTGCCCATGC180.1098766939323648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)