FastQCFastQC Report
Tue 25 Apr 2023
JV173_UniCOI_Tornabene_S043447.1.R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJV173_UniCOI_Tornabene_S043447.1.R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20432
Sequences flagged as poor quality0
Sequence length250
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGACTTCGGGATGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATTGG6133.000195771339076No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATTGG5792.8337901331245106No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATTGG5672.7750587314017228No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATTGG5592.735904463586531No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATTGG5472.677173061863743No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATTGG5232.5597102584181677No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATTGG5192.5401331245105716No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATTGG5072.481401722787784No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATTGG5012.45203602192639No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATTGG4962.427564604541895No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATTGG4822.3590446358653097No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATTGG4782.3394675019577136No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATTGG4552.2268989819890366No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAACAGGTGTTGATAAAGGATTGG4412.158379013312451No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAACAGGTGTTGATAAAGGATTGG4352.129013312451057No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAACAGGTGTTGATAAAGGATTGG4031.9723962411902898No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATTGG3981.947924823805795No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATTGG3981.947924823805795No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATTGG3881.8989819890368052No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAACAGGTGTTGATAAAGGATTGG3771.8451448707909162No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATTGG3711.8157791699295223No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATTGG3701.8108848864526232No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAACAGGTGTTGATAAAGGATTGG3701.8108848864526232No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATTGG3641.7815191855912293No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATTGG3421.6738449490994518No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATTGG3361.644479248238058No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATTGG3331.629796397807361No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATTGG3251.5906421299921691No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATTGG3151.5416992952231792No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAACAGGTGTTGATAAAGGATTGG2821.380187940485513No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAACAGGTGTTGATAAAGGATTGG2761.350822239624119No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAACAGGTGTTGATAAAGGATTGG2711.326350822239624No Hit
TAGACTTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG620.3034455755677369No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG620.3034455755677369No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG610.2985512920908379No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG600.29365700861393895No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG600.29365700861393895No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG600.29365700861393895No Hit
TAGACCTCGGGATGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG570.27897415818324195No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG540.264291307752545No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG540.264291307752545No Hit
TAGACTTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG520.2545027407987471No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG520.2545027407987471No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG510.24960845732184808No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG480.23492560689115116No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG480.23492560689115116No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG470.23003132341425214No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG460.22513703993735315No Hit
TAGACCTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG450.2202427564604542No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG440.2153484729835552No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG430.21045418950665623No Hit
TAGACTTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG430.21045418950665623No Hit
TAGACTTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG400.19577133907595928No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTAAAGAATTGG390.1908770555990603No Hit
TAGACTTCGGGGTGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG390.1908770555990603No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTAAAGAATTGG380.1859827721221613No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG380.1859827721221613No Hit
TAGACCTCGGGATGGCCAAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG370.18108848864526234No Hit
TAGACTTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG370.18108848864526234No Hit
TAGACCTCGGGATGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG300.14682850430696948No Hit
TAGACCTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTAAAGAATTGG300.14682850430696948No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG290.14193422083007046No Hit
TAGACCTCGGGGTGGCCAAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG270.1321456538762725No Hit
TAGACCTCGGGATGGCCGAAAAACCAGAAGAGGTGTTGGTAAAGAATTGG230.11256851996867658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)