FastQCFastQC Report
Tue 25 Apr 2023
JV173_UniCOI_Tornabene_S043447.1.R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJV173_UniCOI_Tornabene_S043447.1.R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20432
Sequences flagged as poor quality0
Sequence length250
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACTGGTTGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC2951.4438136256851997No Hit
GGTACTGGTTGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC2931.4340250587314018No Hit
GGTACTGGTTGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC2521.2333594361785434No Hit
GGTACTGGATGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC2431.1893108848864526No Hit
GGTACTGGTTGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC2331.1403680501174627No Hit
GGTACTGGTTGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC2251.101213782302271No Hit
GGAACTGGTTGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC2251.101213782302271No Hit
GGTACAGGTTGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC2201.076742364917776No Hit
GGTACTGGTTGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC2171.062059514487079No Hit
GGTACAGGTTGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC2151.0522709475332812No Hit
GGAACTGGTTGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC2051.0033281127642912No Hit
GGTACTGGATGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC2030.9935395458104934No Hit
GGTACTGGTTGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1990.9739624119028975No Hit
GGTACAGGTTGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1980.9690681284259985No Hit
GGTACAGGTTGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1930.9445967110415036No Hit
GGTACTGGATGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1910.9348081440877055No Hit
GGTACTGGTTGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1890.9250195771339075No Hit
GGTACTGGTTGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1880.9201252936570086No Hit
GGTACTGGTTGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1870.9152310101801095No Hit
GGAACTGGTTGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1850.9054424432263116No Hit
GGTACAGGTTGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1740.8516053249804229No Hit
GGTACAGGTTGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1680.822239624119029No Hit
GGTACTGGTTGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1650.807556773688332No Hit
GGTACTGGTTGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1650.807556773688332No Hit
GGTACTGGATGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1630.7977682067345341No Hit
GGTACAGGTTGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1610.7879796397807362No Hit
GGTACAGGATGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1600.7830853563038371No Hit
GGAACTGGTTGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1550.7586139389193421No Hit
GGTACTGGATGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1510.7390368050117463No Hit
GGTACAGGATGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1480.7243539545810493No Hit
GGAACAGGTTGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1480.7243539545810493No Hit
GGAACTGGTTGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1470.7194596711041503No Hit
GGAACTGGTTGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1460.7145653876272514No Hit
GGAACTGGTTGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1450.7096711041503524No Hit
GGAACAGGTTGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1450.7096711041503524No Hit
GGTACAGGTTGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1450.7096711041503524No Hit
GGTACAGGTTGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1440.7047768206734534No Hit
GGAACTGGTTGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1420.6949882537196554No Hit
GGAACTGGATGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1420.6949882537196554No Hit
GGTACAGGATGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1400.6851996867658575No Hit
GGTACTGGATGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1390.6803054032889585No Hit
GGAACTGGATGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1390.6803054032889585No Hit
GGTACTGGTTGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC1380.6754111198120595No Hit
GGTACTGGATGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1360.6656225528582616No Hit
GGAACTGGTTGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1350.6607282693813625No Hit
GGTACAGGTTGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1340.6558339859044635No Hit
GGTACTGGATGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1340.6558339859044635No Hit
GGTACTGGTTGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1320.6460454189506656No Hit
GGTACTGGATGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC1310.6411511354737667No Hit
GGTACTGGATGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1260.6166797180892717No Hit
GGTACTGGATGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1260.6166797180892717No Hit
GGAACAGGATGAACTGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1260.6166797180892717No Hit
GGTACTGGATGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1260.6166797180892717No Hit
GGAACAGGTTGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1250.6117854346123728No Hit
GGAACTGGATGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1250.6117854346123728No Hit
GGTACAGGATGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1240.6068911511354738No Hit
GGAACTGGATGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1230.6019968676585749No Hit
GGTACTGGTTGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1220.5971025841816758No Hit
GGTACAGGATGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1190.5824197337509789No Hit
GGTACAGGATGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1180.5775254502740799No Hit
GGTACAGGATGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1170.5726311667971808No Hit
GGTACAGGTTGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC1160.5677368833202818No Hit
GGTACTGGATGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1160.5677368833202818No Hit
GGTACAGGTTGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1140.5579483163664839No Hit
GGTACAGGTTGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1140.5579483163664839No Hit
GGAACTGGTTGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1130.553054032889585No Hit
GGAACAGGTTGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1110.543265465935787No Hit
GGAACAGGTTGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC1100.538371182458888No Hit
GGAACAGGATGAACTGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1090.533476898981989No Hit
GGTACAGGTTGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1090.533476898981989No Hit
GGAACTGGTTGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1080.52858261550509No Hit
GGTACTGGTTGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC1080.52858261550509No Hit
GGTACAGGATGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1070.5236883320281911No Hit
GGAACTGGTTGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1060.5187940485512921No Hit
GGAACAGGTTGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC1050.5138997650743932No Hit
GGAACAGGATGAACTGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1040.5090054815974941No Hit
GGAACAGGTTGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1040.5090054815974941No Hit
GGTACTGGATGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1030.5041111981205951No Hit
GGTACTGGATGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1030.5041111981205951No Hit
GGAACAGGTTGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC1020.49921691464369616No Hit
GGTACAGGATGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC1010.4943226311667972No Hit
GGAACTGGATGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC1010.4943226311667972No Hit
GGAACTGGATGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC1000.48942834768989824No Hit
GGTACAGGATGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC1000.48942834768989824No Hit
GGAACTGGTTGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC990.48453406421299927No Hit
GGAACAGGTTGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC980.47963978073610025No Hit
GGTACAGGTTGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC970.4747454972592013No Hit
GGAACTGGATGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC970.4747454972592013No Hit
GGTACAGGTTGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC970.4747454972592013No Hit
GGAACAGGATGAACAGTTTATCCTCCTCTATCAGGCAACCTAGCCCACGC960.4698512137823023No Hit
GGAACTGGATGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC940.4600626468285043No Hit
GGAACTGGATGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC920.4502740798747063No Hit
GGAACTGGTTGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC920.4502740798747063No Hit
GGAACTGGATGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC900.4404855129209084No Hit
GGAACAGGTTGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC880.4306969459671104No Hit
GGAACTGGATGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC860.42090837901331246No Hit
GGTACAGGATGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC850.41601409553641344No Hit
GGAACTGGTTGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC820.40133124510571655No Hit
GGAACTGGATGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC810.3964369616288176No Hit
GGTACAGGATGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC810.3964369616288176No Hit
GGAACAGGATGAACAGTTTATCCCCCTCTATCAGGCAACCTAGCCCACGC790.38664839467501955No Hit
GGAACTGGATGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC790.38664839467501955No Hit
GGAACAGGATGAACTGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC760.3719655442443226No Hit
GGAACTGGTTGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC750.36707126076742363No Hit
GGAACAGGTTGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC730.3572826938136257No Hit
GGAACAGGATGAACTGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC730.3572826938136257No Hit
GGTACAGGATGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC730.3572826938136257No Hit
GGAACAGGATGAACTGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC720.3523884103367267No Hit
GGAACAGGTTGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC720.3523884103367267No Hit
GGAACAGGATGAACTGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC700.34259984338292876No Hit
GGTACAGGATGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC690.33770555990602974No Hit
GGTACTGGATGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC670.32791699295223176No Hit
GGTACAGGATGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC670.32791699295223176No Hit
GGAACAGGTTGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC660.3230227094753328No Hit
GGAACAGGATGAACAGTTTACCCCCCTCTATCAGGCAACCTAGCCCACGC650.31812842599843383No Hit
GGAACTGGATGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC620.3034455755677369No Hit
GGAACAGGATGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC620.3034455755677369No Hit
GGAACTGGATGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC620.3034455755677369No Hit
GGAACAGGTTGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC620.3034455755677369No Hit
GGAACAGGTTGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC600.29365700861393895No Hit
GGTACAGGATGAACAGTATATCCTCCTCTATCAGGCAACCTAGCCCACGC560.274079874706343No Hit
GGAACAGGATGAACAGTATACCCCCCTCTATCAGGCAACCTAGCCCACGC540.264291307752545No Hit
GGAACAGGATGAACAGTTTACCCTCCTCTATCAGGCAACCTAGCCCACGC530.25939702427564604No Hit
GGAACAGGATGAACTGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC500.24471417384494912No Hit
GGAACTGGATGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC460.22513703993735315No Hit
GGAACAGGATGAACAGTATATCCCCCTCTATCAGGCAACCTAGCCCACGC430.21045418950665623No Hit
GGAACAGGTTGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC380.1859827721221613No Hit
GGAACAGGATGAACAGTATACCCTCCTCTATCAGGCAACCTAGCCCACGC330.1615113547376664No Hit
GGTACTGGTTGAACTGTTTATCCTCCCTTAGCGGGCAATCTTGCCCATGC230.11256851996867658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)