@HD VN:1.4 SO:coordinate @SQ SN:000000F LN:686 @RG ID:rg SM:sample @CO The alignment on the first read overhangs the reference by two bases ST-E00128:308:HHVVLALXX:8:1217:16001:6565 147 000000F 532 60 6M1D117M5D28M = 195 -494 CAGGTATGGTCTGGACAGGAGAGAAACTGTGGATGTCTGTCAACACAACAGAAAAAGTGACTGATTAGTTAGATGTAACTAATTTCAACTATCTAGTCTTCCTCTAAAGACCCCTACTTTAACACAAATTACATCTAGATATTTGTTATTT 7AFAAF<)7<<7<7FA