RsubreadUsersGuide View Rsubread Users Guide align Align Sequence Reads to a Reference Genome via Seed-and-Vote atgcContent Calculate Percentages of Nucleotides in Reads buildindex Build Index for a Reference Genome cellCounts Map and quantify single-cell RNA-seq data generated by 10X Genomics detectionCall Determine Detection P Values for Each Gene in an RNA-seq Dataset detectionCallAnnotation Generate Annotation Data Used for Calculating Detection P Values exactSNP Accurately and Efficiently call SNPs featureCounts Count Reads by Genomic Features findCommonVariants Finding the Common Variants Among All Input VCF Files flattenGTF Flatten Features in GTF or GFF Annotation Files getInBuiltAnnotation Retrieve inbuilt gene annotations processExons Obtain Chromosomal Coordiates of Each Exon Using NCBI Annotation promoterRegions Generate Annotation for Promoter Regions of Genes propmapped Calculate the Proportion of Mapped Reads or Fragments in SAM/BAM Files qualityScores Extract Quality Score Data in a Sequencing Read Dataset removeDupReads Remove Sequencing Reads Mapped to Identical Locations repair Re-Order Paired-End Reads to Place Reads sam2bed Convert a SAM Format File to a BED File simReads Generate Simulated Reads from a Set of Transcripts sublong Align Long Sequence Reads to a Reference Genome via Seed-and-Vote txUnique Count Number of Bases Unique to Each Transcript