DESeq Differential expression analysis based on the Negative Binomial (a.k.a. Gamma-Poisson) distribution DESeq2-package DESeq2 package for differential analysis of count data DESeqDataSet-class DESeqDataSet object and constructors DESeqResults-class DESeqResults object and constructor DESeqTransform-class DESeqTransform object and constructor coef.DESeqDataSet Extract a matrix of model coefficients/standard errors collapseReplicates Collapse technical replicates in a RangedSummarizedExperiment or DESeqDataSet counts,DESeqDataSet-method Accessors for the 'counts' slot of a DESeqDataSet object. design,DESeqDataSet-method Accessors for the 'design' slot of a DESeqDataSet object. dispersionFunction Accessors for the 'dispersionFunction' slot of a DESeqDataSet object. dispersions Accessor functions for the dispersion estimates in a DESeqDataSet object. estimateBetaPriorVar Steps for estimating the beta prior variance estimateDispersions,DESeqDataSet-method Estimate the dispersions for a DESeqDataSet estimateDispersionsGeneEst Low-level functions to fit dispersion estimates estimateSizeFactors,DESeqDataSet-method Estimate the size factors for a 'DESeqDataSet' estimateSizeFactorsForMatrix Low-level function to estimate size factors with robust regression. fpkm FPKM: fragments per kilobase per million mapped fragments fpm FPM: fragments per million mapped fragments integrateWithSingleCell Integrate bulk DE results with Bioconductor single-cell RNA-seq datasets lfcShrink Shrink log2 fold changes makeExampleDESeqDataSet Make a simulated DESeqDataSet nbinomLRT Likelihood ratio test (chi-squared test) for GLMs nbinomWaldTest Wald test for the GLM coefficients normTransform Normalized counts transformation normalizationFactors Accessor functions for the normalization factors in a DESeqDataSet object. normalizeGeneLength Normalize for gene length plotCounts Plot of normalized counts for a single gene plotDispEsts Plot dispersion estimates plotMA MA-plot from base means and log fold changes plotPCA Sample PCA plot for transformed data plotSparsity Sparsity plot priorInfo Accessors for the 'priorInfo' slot of a DESeqResults object. replaceOutliers Replace outliers with trimmed mean results Extract results from a DESeq analysis rlog Apply a 'regularized log' transformation show,DESeqResults-method Show method for DESeqResults objects sizeFactors,DESeqDataSet-method Accessor functions for the 'sizeFactors' information in a DESeqDataSet object. summary,DESeqResults-method Summarize DESeq results unmix Unmix samples using loss in a variance stabilized space varianceStabilizingTransformation Apply a variance stabilizing transformation (VST) to the count data vst Quickly estimate dispersion trend and apply a variance stabilizing transformation