FastQCFastQC Report
Thu 20 Apr 2023
SRR7725722_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7725722_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21571941
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACAAAGTTAGTATAAATAAATTTTAGTA1138560.5277967337292457No Hit
GCATAATTGTTTTGTGAAAAGTGGACTTGAACAAAGTTAGTATAAATAAA729110.3379899843041477No Hit
GTGCATAATTGTTTTGTGAAAAGTGGACTTGAACAAAGTTAGTATAAATA645190.29908759717078776No Hit
CGTTAACGTGAGCGTCCTAAGGTAGCGCGATAATTTGCTTTTCAATTGAA563730.26132558029896336No Hit
CTTGAACAAAGTTAGTATAAATAAATTTTAGTACCTTTTGCATAAGGGTT536750.2488185926338293No Hit
CGGCAAACTAAACTTCTCGACTGTTTAACAAAAACATTTCCTTTTGATGT426750.19782642646760437No Hit
CTTTAAACTGGCTATTAGGTATTAGTGTATTTTAACTAAAACCAAACGGA421210.1952582755534145No Hit
GCTAAATAAAGGTTTTTAAATGAACTCGGCAAACTAAACTTCTCGACTGT384610.17829179117447058No Hit
CAAAAACATTTCCTTTTGATGTAAGTAAAAGGTAGTCCCTGCCCAGTGCA381260.17673884793213557No Hit
CGATAATTTGCTTTTCAATTGAAGGATGGTATGAAAGGGTTAACGAAGAA380820.17653487926747063No Hit
CGAAGAAGGTGCTGTGTCTAAAAATTCAATTTAAACTAACTTTAAGGTGA377620.17505147079718048No Hit
CTAAACTTCTCGACTGTTTAACAAAAACATTTCCTTTTGATGTAAGTAAA373000.172909799818199No Hit
GTGAAATTGATGTTTAAAAAATTTTTGGTTTCTATAGGCTTTAAACTGGC364160.1688118839190224No Hit
GTGTATTTTAACTAAAACCAAACGGAGAAAAAGATTAATTGGGGTCATCA355730.1649040297301017No Hit
CGGAGGGTCAGTAATTATGATAAAAACGAGTAAAAATATTATTTTTTTGG345460.16014321567076417No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA341830.15846047418727874No Hit
ATTTGATTAAACTAAAGAATGGCGGCTAGATACTATTCGCGCTTTTAGAT336600.15603602846864822No Hit
GTAGAAATAAGTCTTCTACTATCATATTAATCTTACTAGTATTTCCTAAC333040.1543857365454504No Hit
CGTGCATAATTGTTTTGTGAAAAGTGGACTTGAACAAAGTTAGTATAAAT332450.15411223310874067No Hit
CTTCTCGACTGTTTAACAAAAACATTTCCTTTTGATGTAAGTAAAAGGTA316480.14670909771169874No Hit
GAAAGGGTTAACGAAGAAGGTGCTGTGTCTAAAAATTCAATTTAAACTAA312580.14490119363853257No Hit
GTAAAAGGTAGTCCCTGCCCAGTGCAACTAGAGTAATGTCTGTTGTAAAC311110.14421975287249303No Hit
CGGCTAGATACTATTCGCGCTTTTAGATATCTGGTTGGCTTAAAAAAATG309860.14364029643878592No Hit
GTCTGTTGTAAACGGCGGCGTTAACGTGAGCGTCCTAAGGTAGCGCGATA290410.1346239543303034No Hit
ATTGTTTTGTGAAAAGTGGACTTGAACAAAGTTAGTATAAATAAATTTTA278860.1292697768828498No Hit
CGTGAGCGTCCTAAGGTAGCGCGATAATTTGCTTTTCAATTGAAGGATGG277900.12882475434176274No Hit
AGCTACTCTAGGGATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAG265810.12322025171494766No Hit
CATAATTGTTTTGTGAAAAGTGGACTTGAACAAAGTTAGTATAAATAAAT265480.12306727521644899No Hit
CAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTC255100.11825546899094522No Hit
GTGGACTTGAACAAAGTTAGTATAAATAAATTTTAGTACCTTTTGCATAA254450.11795415164541753No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG254160.1178197177527975No Hit
ATCTGGTTGGCTTAAAAAAATGTGTAAGCATTTTCTTTAACTACTTAAGG234200.1085669574193625No Hit
ATTTTCTTTAACTACTTAAGGGGTAAAATTTTTTGGGTTAAGCTGAAAAA233630.10830272528559205No Hit
CTTACTAGTATTTCCTAACTTTATATGTGTGGCTAGCTACTCTAGGGATA233200.1081033922723968No Hit
GGCTAAATAAAGGTTTTTAAATGAACTCGGCAAACTAAACTTCTCGACTG227840.10561868308466077No Hit
CCTAAGGTAGCGCGATAATTTGCTTTTCAATTGAAGGATGGTATGAAAGG220800.10235518445002235No Hit
AATTGTTTTGTGAAAAGTGGACTTGAACAAAGTTAGTATAAATAAATTTT219900.10193797581775327No Hit
CGACAAGACCCTATGAAGCTTTATCTTAATTGGAGCTCTCAGGCTCTTAT218500.10128898461200131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)