FastQCFastQC Report
Thu 20 Apr 2023
SRR7725722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7725722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21571941
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTTTATCATAATTACTGACCCTCCGATCATAGGCTTATTTTTAAA1029270.47713369881736656No Hit
GCCGTTCTGAACTCAGCTCATGTTAGATTTTAAAGGGCGAACAGACCCAC1017040.4714642970699763No Hit
CTTTCGTACAAAATTAGCTCATTTCAAAAAGAGGATAGAAACTGAACTAG982800.45559182643787133No Hit
CCCTTAAGTAGTTAAAGAAAATGCTTACACATTTTTTTAAGCCAACCAGA922030.4274209724567669No Hit
GTCCTTTCGTACAAAATTAGCTCATTTCAAAAAGAGGATAGAAACTGAAC917280.42521903800867994No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAGAGCTCCAATTAAGATAAAGCT870840.40369107258359366No Hit
CCCTAGAGTAGCTAGCCACACATATAAAGTTAGGAAATACTAGTAAGATT858550.3979938569273854No Hit
CCTTAAGTAGTTAAAGAAAATGCTTACACATTTTTTTAAGCCAACCAGAT753860.3494632216915483No Hit
CTCGTTTTTATCATAATTACTGACCCACCGATCATAAGCTTATTTTTAGA639850.296612159285991No Hit
CGAGAAGTTTAGTTTGCCGAGTTCATTTAAAAACCTTTATTTAGCCGTCA636620.29511484386129183No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATACCTAATAGCCAGTTTAAA623530.2890467760875111No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAAATTTTTTAAACATCAATTTCAC619390.2871276163790732No Hit
GTCGAGAAGTTTAGTTTGCCGAGTTCATTTAAAAACCTTTATTTAGCCGT618900.28690046945706No Hit
GCTCATTTCAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAA566430.2625772061957707No Hit
GTCTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTT555510.25751507479090546No Hit
GCACCTTCTTCGTTAACCCTTTCATACCATCCTTCAATTGAAAAGCAAAT553640.2566482079660796No Hit
CCACGATTTCCTAACTCAAAAAAATAACTCTCTTTTATTCGGGAAAATTA550920.255387310766333No Hit
CTTAAGTAGTTAAAGAAAATGCTTACACATTTTTTTAAGCCAACCAGATA550230.25506745081492666No Hit
CTCATTTCAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC527030.24431273940532286No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT520580.24132274420739422No Hit
CACACATATAAAGTTAGGAAATACTAGTAAGATTAATATGATAGTAGAAG501570.2325103707635766No Hit
CCCCAATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATAC477190.2212086524805533No Hit
CTCACGTTAACGCCGCCGTTTACAACAGACATTACTCTAGTTGCACTGGG477190.2212086524805533No Hit
CCCAATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATACC474300.2198689492058225No Hit
CCAACATCGAGGTCGCAATCTTCCCCTCCAATACCATCTTTCGGGAGAAA398480.18472143976288458No Hit
CACCATTTTTCAGCTTAACCCAAAAAATTTTACCCCTTAAGTAGTTAAAG394850.18303869827939914No Hit
CTCCGATCATAGGCTTATTTTTAAATAAATGATGACCCCAATTAATCTTT392730.18205594016783191No Hit
CCTAGAGTAGCTAGCCACACATATAAAGTTAGGAAATACTAGTAAGATTA380920.17658123578216722No Hit
CGAGAAGTTTAGTTTGCCGAGTTCATTTAAAAACCTTTATTTAGCCGTTA374920.17379984490037312No Hit
CGTCAATCTTTTAAGGTAAAACAAAACAGTGATACCAAAAAAATAATATT369970.17150519742289302No Hit
GTTCTGAACTCAGCTCATGTTAGATTTTAAAGGGCGAACAGACCCACCAT355560.16482522365511754No Hit
CGCCGTTCTGAACTCAGCTCATGTTAGATTTTAAAGGGCGAACAGACCCA354100.16414841854054765No Hit
CTCATGTTAGATTTTAAAGGGCGAACAGACCCACCATCAGAAGCTTCTAC337690.15654131447884082No Hit
GCCACACATATAAAGTTAGGAAATACTAGTAAGATTAATATGATAGTAGA323670.150042131118382No Hit
CCCTTTCATACCATCCTTCAATTGAAAAGCAAATTATCGCGCTACCTTAG321390.14898520258330022No Hit
GCCGAGTTCATTTAAAAACCTTTATTTAGCCGTCAATCTTTTAAGGTAAA316610.14676936118080428No Hit
CTTCAATTGAAAAGCAAATTATCGCGCTACCTTAGGACGCTCACGTTAAC303840.14084963425405253No Hit
ACCATTTTTCAGCTTAACCCAAAAAATTTTACCCCTTAAGTAGTTAAAGA271440.12583012349236447No Hit
GGGTCTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAG264930.12281231438561788No Hit
CCATTTTTCAGCTTAACCCAAAAAATTTTACCCCTTAAGTAGTTAAAGAA261280.12112030159919314No Hit
CTTTCATACCATCCTTCAATTGAAAAGCAAATTATCGCGCTACCTTAGGA257270.11926140535986075No Hit
CACAGATTTGTGACAAAGCATTGCTTTAGCGAAAAAGTCCTTTCGTACAA257080.11917332798193726No Hit
CGTTAATCTTTTAAGGTAAAACAAAACAGTGATACCAAAAAAATAATATT254550.1180005081601141No Hit
CCACCATCAGAAGCTTCTACGCCTCTAGGATATCTAAAGCCAACATCGAG253980.11773627602634365No Hit
CACCATCAGAAGCTTCTACGCCTCTAGGATATCTAAAGCCAACATCGAGG247080.11453767651228047No Hit
CTCTAGGATATCTAAAGCCAACATCGAGGTCGCAATCTTCCCCTCCAATA245770.11393040616975543No Hit
GCCGTCAATCTTTTAAGGTAAAACAAAACAGTGATACCAAAAAAATAATA243500.11287811328614333No Hit
GTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTAAATTGA241910.1121410447024679No Hit
GTACAAAATTAGCTCATTTCAAAAAGAGGATAGAAACTGAACTAGCTCAC240030.11126954222617241No Hit
GGCCTCTTCACCTTAAAGTTAGTTTAAATTGAATTTTTAGACACAGCACC238460.11054174494543628No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA229820.10653654207565282No Hit
CATCAAAATCGTATAAGAGCCTGAGAGCTCCAATTAAGATAAAGCTTCAT226610.10504849795389297No Hit
CCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTAAATTGAAT225810.10467764583632043No Hit
GAGAAGTTTAGTTTGCCGAGTTCATTTAAAAACCTTTATTTAGCCGTCAA220350.1021465801338878No Hit
CTTAAAGTTAGTTTAAATTGAATTTTTAGACACAGCACCTTCTTCGTTAA219310.10166447238104351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)