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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.12

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2024-06-05, 04:54 based on data in: /home/shared/8TB_HDD_03/sr320/github/grace-L-byssus/output/13-Hisat


        General Statistics

        Showing 131/131 rows.
        Sample Name% Aligned
        A1-T001F_S100_hisat
        72.1%
        A1-T030F_S196_hisat
        68.0%
        A10-T015F_S172_hisat
        70.7%
        A11-T027F_S180_hisat
        69.9%
        A12-T021G_S188_hisat
        64.1%
        A2-T009F_S108_hisat
        73.6%
        A2-T039F_S204_hisat
        67.7%
        A3-T005G_S116_hisat
        62.1%
        A3-T035G_S212_hisat
        64.8%
        A4-T002FX_S220_hisat
        71.8%
        A4-T126F_S124_hisat
        70.0%
        A5-T010FX_S228_hisat
        71.8%
        A5-T134F_S132_hisat
        69.1%
        A6-T130G_S140_hisat
        62.8%
        A7-T046F_S148_hisat
        67.3%
        A8-T110F_S156_hisat
        69.2%
        A9-T055G_S164_hisat
        63.2%
        B1-T002F_S101_hisat
        72.0%
        B1-T031F_S197_hisat
        69.0%
        B10-T016F_S173_hisat
        68.2%
        B11-T118F_S181_hisat
        71.1%
        B12-T023G_S189_hisat
        64.1%
        B2-T010F_S109_hisat
        72.3%
        B2-T040F_S205_hisat
        65.6%
        B3-T006G_S117_hisat
        63.6%
        B3-T036G_S213_hisat
        63.8%
        B4-T003FX_S221_hisat
        70.8%
        B4-T127F_S125_hisat
        70.0%
        B5-T011FX_S229_hisat
        70.1%
        B5-T135F_S133_hisat
        67.8%
        B6-T131G_S141_hisat
        63.2%
        B7-T049F_S149_hisat
        67.2%
        B8-T111F_S157_hisat
        69.1%
        B9-T056G_S165_hisat
        63.2%
        C1-T003F_S102_hisat
        72.8%
        C1-T033F_S198_hisat
        68.2%
        C10-T017F_S174_hisat
        68.0%
        C11-T119F_S182_hisat
        70.8%
        C12-T025G_S190_hisat
        60.8%
        C2-T011F_S110_hisat
        71.7%
        C2-T041F_S206_hisat
        68.3%
        C3-T007G_S118_hisat
        66.7%
        C3-T037G_S214_hisat
        63.4%
        C4-T004FX_S222_hisat
        70.7%
        C4-T128F_S126_hisat
        69.4%
        C5-T012FX_S230_hisat
        70.2%
        C5-T136F_S134_hisat
        68.8%
        C6-T132G_S142_hisat
        64.2%
        C7-T051F_S150_hisat
        79.0%
        C8-T112F_S158_hisat
        69.7%
        C9-T057G_S166_hisat
        65.0%
        D1-T004F_S103_hisat
        72.4%
        D1-T034F_S199_hisat
        71.0%
        D10-T019F_S175_hisat
        69.5%
        D11-T014G_S183_hisat
        64.8%
        D12-T026G_S191_hisat
        63.6%
        D2-T012F_S111_hisat
        70.8%
        D2-T029G_S207_hisat
        62.5%
        D3-T008G_S119_hisat
        66.1%
        D3-T038G_S215_hisat
        63.1%
        D4-T005FX_S223_hisat
        70.8%
        D4-T129F_S127_hisat
        69.4%
        D5-T137F_S135_hisat
        68.5%
        D6-T133G_S143_hisat
        64.4%
        D7-T052F_S151_hisat
        67.9%
        D8-T046G_S159_hisat
        63.3%
        D9-T058G_S167_hisat
        64.4%
        E1-T005F_S104_hisat
        72.1%
        E1-T035F_S200_hisat
        68.9%
        E10-T021F_S176_hisat
        67.3%
        E11-T015G_S184_hisat
        64.7%
        E12-T027G_S192_hisat
        63.5%
        E2-T001G_S112_hisat
        67.0%
        E2-T030G_S208_hisat
        62.3%
        E3-T009G_S120_hisat
        66.4%
        E3-T039G_S216_hisat
        63.3%
        E4-T006FX_S224_hisat
        70.8%
        E4-T130F_S128_hisat
        70.6%
        E5-T126G_S136_hisat
        63.0%
        E6-T134G_S144_hisat
        63.0%
        E7-T055F_S152_hisat
        68.3%
        E8-T047G_S160_hisat
        64.3%
        E9-T110G_S168_hisat
        63.5%
        F1-T006F_S105_hisat
        74.2%
        F1-T036F_S201_hisat
        69.7%
        F10-T023F_S177_hisat
        70.4%
        F11-T016G_S185_hisat
        61.8%
        F12-T118G_S193_hisat
        65.2%
        F2-T002G_S113_hisat
        67.2%
        F2-T031G_S209_hisat
        63.5%
        F3-T010G_S121_hisat
        66.1%
        F3-T040G_S217_hisat
        63.4%
        F4-T007FX_S225_hisat
        73.8%
        F4-T131F_S129_hisat
        69.6%
        F5-T127G_S137_hisat
        64.2%
        F6-T135G_S145_hisat
        63.4%
        F7-T056F_S153_hisat
        68.2%
        F8-T049G_S161_hisat
        63.1%
        F9-T111G_S169_hisat
        63.7%
        G1-T007F_S106_hisat
        72.1%
        G1-T037F_S202_hisat
        71.8%
        G10-T025F_S178_hisat
        66.8%
        G11-T017G_S186_hisat
        64.2%
        G12-T119G_S194_hisat
        64.5%
        G2-T003G_S114_hisat
        65.7%
        G2-T033G_S210_hisat
        61.4%
        G3-T011G_S122_hisat
        65.9%
        G3-T041G_S218_hisat
        63.6%
        G4-T008FX_S226_hisat
        70.1%
        G4-T132F_S130_hisat
        70.8%
        G5-T128G_S138_hisat
        64.0%
        G6-T136G_S146_hisat
        63.8%
        G7-T057F_S154_hisat
        70.6%
        G8-T051G_S162_hisat
        63.8%
        G9-T112G_S170_hisat
        63.2%
        H1-T008F_S107_hisat
        70.5%
        H1-T038F_S203_hisat
        68.7%
        H10-T026F_S179_hisat
        70.1%
        H11-T019G_S187_hisat
        64.9%
        H12-T029F_S195_hisat
        72.5%
        H2-T004G_S115_hisat
        65.7%
        H2-T034G_S211_hisat
        62.7%
        H3-T001FX_S219_hisat
        71.8%
        H3-T012G_S123_hisat
        64.7%
        H4-T009FX_S227_hisat
        72.4%
        H4-T133F_S131_hisat
        69.4%
        H5-T129G_S139_hisat
        62.3%
        H6-T137G_S147_hisat
        62.1%
        H7-T058F_S155_hisat
        67.9%
        H8-T052G_S163_hisat
        64.1%
        H9-T014F_S171_hisat
        71.0%

        Bowtie 2 / HiSAT2

        Bowtie 2 and HISAT2 are fast and memory-efficient tools for aligning sequencing reads against a reference genome. Unfortunately both tools have identical log output by default, so it is impossible to distiguish which tool was used. .DOI: 10.1038/nmeth.1923; 10.1038/nmeth.3317; 10.1038/s41587-019-0201-4.

        Single-end alignments

        This plot shows the number of reads aligning to the reference in different ways.

        There are 3 possible types of alignment:

        • SE mapped uniquely: Read has only one occurence in the reference genome.
        • SE multimapped: Read has multiple occurence.
        • SE not aligned: Read has no occurence.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).