Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_9_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_9_R2.fastp-trim.20201202.fq.gz

Data processed at 23:58 on 2021-03-12


Alignment Stats

Sequence pairs analysed in total 73776588
Paired-end alignments with a unique best hit 45591578
Pairs without alignments under any condition 16002262
Pairs that did not map uniquely 12182748
Genomic sequence context not extractable (edges of chromosomes) 120

Cytosine Methylation

Total C's analysed 1595529090
Methylated C's in CpG context 21006842
Methylated C's in CHG context 1277934
Methylated C's in CHH context 5350483
Methylated C's in Unknown context 49317
Unmethylated C's in CpG context 188873033
Unmethylated C's in CHG context 281019136
Unmethylated C's in CHH context 1098001662
Unmethylated C's in Unknown context 3841672
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11364394 original top strand
CTOT 11385517 complementary to original top strand
CTOB 11430000 complementary to original bottom strand
OB 11411547 original bottom strand

Deduplication

Alignments analysed 45591458
Unique alignments 37592253
Duplicates removed 7999205
Duplicated alignments were found at 6741104 different positions

Cytosine Methylation after Extraction

Total C's analysed 903220750
Methylated C's in CpG context 11490959
Methylated C's in CHG context 711903
Methylated C's in CHH context 3019557
Unmethylated C's in CpG context 105465948
Unmethylated C's in CHG context 157214343
Unmethylated C's in CHH context 625318040
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot