Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_8_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_8_R2.fastp-trim.20201202.fq.gz

Data processed at 23:58 on 2021-03-12


Alignment Stats

Sequence pairs analysed in total 90605411
Paired-end alignments with a unique best hit 55543581
Pairs without alignments under any condition 20159676
Pairs that did not map uniquely 14902154
Genomic sequence context not extractable (edges of chromosomes) 132

Cytosine Methylation

Total C's analysed 1919611382
Methylated C's in CpG context 25390230
Methylated C's in CHG context 1605629
Methylated C's in CHH context 6877825
Methylated C's in Unknown context 64253
Unmethylated C's in CpG context 221478350
Unmethylated C's in CHG context 332078494
Unmethylated C's in CHH context 1332180854
Unmethylated C's in Unknown context 4697286
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 13773128 original top strand
CTOT 13966090 complementary to original top strand
CTOB 13998198 complementary to original bottom strand
OB 13806033 original bottom strand

Deduplication

Alignments analysed 55543449
Unique alignments 44096319
Duplicates removed 11447130
Duplicated alignments were found at 9375294 different positions

Cytosine Methylation after Extraction

Total C's analysed 1036370828
Methylated C's in CpG context 13257071
Methylated C's in CHG context 856075
Methylated C's in CHH context 3718164
Unmethylated C's in CpG context 118387681
Unmethylated C's in CHG context 177358593
Unmethylated C's in CHH context 722793244
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot