Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_7_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_7_R2.fastp-trim.20201202.fq.gz

Data processed at 23:58 on 2021-03-12


Alignment Stats

Sequence pairs analysed in total 66080050
Paired-end alignments with a unique best hit 40370046
Pairs without alignments under any condition 15234187
Pairs that did not map uniquely 10475817
Genomic sequence context not extractable (edges of chromosomes) 110

Cytosine Methylation

Total C's analysed 1403343205
Methylated C's in CpG context 18233544
Methylated C's in CHG context 1194392
Methylated C's in CHH context 5127016
Methylated C's in Unknown context 46389
Unmethylated C's in CpG context 161678658
Unmethylated C's in CHG context 242953310
Unmethylated C's in CHH context 974156285
Unmethylated C's in Unknown context 3481561
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 10080295 original top strand
CTOT 10080848 complementary to original top strand
CTOB 10101750 complementary to original bottom strand
OB 10107043 original bottom strand

Deduplication

Alignments analysed 40369936
Unique alignments 33703944
Duplicates removed 6665992
Duplicated alignments were found at 5688018 different positions

Cytosine Methylation after Extraction

Total C's analysed 815274441
Methylated C's in CpG context 10242157
Methylated C's in CHG context 682349
Methylated C's in CHH context 2962714
Unmethylated C's in CpG context 93016354
Unmethylated C's in CHG context 139619354
Unmethylated C's in CHH context 568751513
Percentage methylation (CpG context) 9.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot